Influence of chemical composition of soil, climatic, fertilization and phytohormons for morphometrical parameters, quantitative and qualitative composition of essential oil, density of stomata and glandular trichomes, size of glandular trichomes of plants from genus Thymus. Research do at the Field collection of Botany institute Nature Research Centre and in the natural habitats. In the fertilization experiments by individual methodology in every cases done fertilization experiments, the soil take for chemical analysis. Before cut off the raw material do morphometrical measurements. Quantitative analysis of essential oil is done by hydrodistilation method, qualitative analysis by – GC– FID and GC– MS. Anatomical investigation of stomata and glandular trichomes are done by imprint method used light microscope.