Research interests

Identification and classification of phytoplasmas (plant pathogenic bacteria), assessment of their biodiversity and molecular evolutionary relationships based on 16S rDNA and other genetic markers.  Investigation of phytoplasmal diseases and insect vectors; metagenomic investigation of plant endophytic bacterial microbiomes.  Experience in genome libraries data and NGS technology-based data analysis. Methodology.  DNA, RNA, and plasmid DNA extraction; polymerase chain reaction (PCR), RT-PCR, restriction fragments length polymorphism (RFLP) analysis; DNA electrophoresis (agarose, polyacrylamide, Pulse Field); molecular cloning techniques and sequence analysis.  Bioinformatics tools. LaserGene DNASTAR and Geneious Prime softwares (putative restriction site analysis, sequence alignment, primer designing etc.); phylogenetic analysis (ClustalX, TreeView. MegaX softwares); pDraw, iPhyClassifier softwares; sequence analysis using NCBI GeneBank database; Keywords. Phytoplasmas, classification, phylogeny, marker genes, plant diseases, phytoplasma vectors, bacterial microbiome, control of diseases, bacteria of class Mollicutes, plant viruses.

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